INTRODUCTION:
Albumin is the most abundant protein constituent of serum. It is synthesized in the liver and is noted for its ability of configuration changes. This steric affinity allows the albumin molecule to serve as carrier of many substances such as bilirubin, fatty acids, uric acid, various drugs and antibiotics. Albumin also functions in the maintenance of proper osmotic pressure. Elevated serum albumin levels are associated with possible dehydration. Low serum, albumin levels are indicative of potential malnutrition, liver disease, kidney disorders and rheumatoid arthritis. Earlier, salt fractionation methods used to determine serum albumin were too laborious to perform and hence it is replaced by azo dye methods. The use of bromcresol green in the reaction has become the preferred method because of its negligible interference for hemolysis, bilirubin and salicylates.
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