The hCG card is based on the principle of immuno-chromatography. Monoclonal antibodies against hCG are conjugated with the colloidal gold reagent and impregnated on the absorbent pad. The reaction zone constituting the nitrocellulose membrane is coated with polyclonal antibodies against hCG and ant-hCG antibody in the test zone and control zone respectively. The hCG card when dipped into the specimen sample, flows through the sample pad to the conjugate pad. The hCG, if present in the sample binds to the conjugate and this complex moves further by capillary action and binds to the anti hCG antibody forming a purple test line. The excess of the complex moves further where it binds with the polyclonal anti hCG antibodies to form a control line.
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