Bordetella pertussis primer and probe have been designed for the specific and exclusive in vitro quantification of Bordetella pertussis. The target sequence (PNR1 gene) is highly conserved and has previously been shown to be a good genetic marker for Bordetella pertussis. The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis