INTRODUCTION
Creatine Kinase (CK) plays an important role in the energy-storing mechanism of tissue by catalyzing the reversible reaction between creatine and ATP to form creatine phosphate and ADP. CK is distributed in various organs; the highest activities (in decreasing order) are skeletal muscle, heart, and brain. Thus, determination of CK is an aid in diagnosing muscular dystrophy and other diseases of the skeletal muscles, myocardial infarction, hypothyroidism, renal diseases, and/or dysfunction. The early procedure for determining CK was based on the rate of ATP formation. A modified method was described by Nielson by adding a sulfhydryl compound and AMP to assure maximum CK activity and inhibit adenylate kinase activity. Optimized conditions for measuring CK were published by Szasz in 1976 as well as by the Scandinavian committee on enzyme. The above procedure was modified again in 1979 to include EDTA. The present reagent is a modification of the above revision.
Product Features:
Enquiry
