Human HTRA1(High Temperature Requirement Factor A1) ELISA Kit

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CATALOGUE ID - ELK6091

• Detectionrange:31.25-2000 pg/mL
• Alternative Names:HtrA; L56; ORF480; PRSS11; HtrA Serine Peptidase 1; Protease,Serine,11; IGFBP5-Protease; High-temperature requirement A serine peptidase 1
• Assay Type:Sandwich
• Sensitivity:13.2 pg/mL
• Standard:2000 pg/mL
• Sample Type:Serum, plasma and other biological fluids
• Assay Length:3.5h
• Research Area:Enzyme & Kinase;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Temperature Requirement Factor A1(HTRA1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to High Temperature Requirement Factor A1(HTRA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Temperature Requirement Factor A1(HTRA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  High Temperature Requirement Factor A1(HTRA1) in the samples is then determined by comparing the OD of the samples to the standard curve.