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Human SEMA3A(Semaphorin 3A) ELISA Kit

Delivery Time:

Atleast 7 days from order confirmation date
Qty :
  • ELK6103
  • Detectionrange:0.16-10 ng/mL
  • Alternative Names:SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
  • Assay Type:Sandwich
  • Sensitivity:0.057 ng/mL
  • Standard:10 ng/mL
  • Sample Type:serum, plasma, tissue homogenates and other biological fluids
  • Assay Length:3.5h
  • Research Area:CD & Adhesion molecule;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A(SEMA3A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Semaphorin 3A(SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3A(SEMA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Semaphorin 3A(SEMA3A) in the samples is then determined by comparing the OD of the samples to the standard curve.