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ELK6108-Human INPP4A(Inositol Polyphosphate-4-Phosphatase Type I 107kDa) ELISA Kit

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Atleast 7 days from order confirmation date
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  • Detectionrange:78.13-5000 pg/mL
  • Alternative Names:INPP4; Inositol polyphosphate 4-phosphatase type I; Type I inositol 3,4-bisphosphate 4-phosphatase
  • Assay Type:Sandwich
  • Sensitivity:30 pg/mL
  • Standard:5000 pg/mL
  • Sample Type:Tissue homogenates, cell lysates and other biological fluids
  • Assay Length:3.5h
  • Research Area:Signal transduction;Enzyme & Kinase;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa(INPP4A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa(INPP4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-4-Phosphatase Type I 107kDa(INPP4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Inositol Polyphosphate-4-Phosphatase Type I 107kDa(INPP4A) in the samples is then determined by comparing the OD of the samples to the standard curve.