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ELK6130-Human CLDN9(Claudin 9) ELISA Kit

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Atleast 7 days from order confirmation date
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  • Detectionrange:78.13-5000 pg/mL
  • Alternative Names:CLDN9; Claudin-9
  • Assay Type:Sandwich
  • Sensitivity:33 pg/mL
  • Standard:5000 pg/mL
  • Sample Type:Tissue homogenates and other biological fluids.
  • Assay Length:3.5h
  • Research Area:Metabolic pathway;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 9(CLDN9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Claudin 9(CLDN9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 9(CLDN9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Claudin 9(CLDN9) in the samples is then determined by comparing the OD of the samples to the standard curve.