ELK6143-Human IL1F9(Interleukin 1 Family, Member 9) ELISA Kit

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• Detectionrange:15.63-1000 pg/mL
• Alternative Names:IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2
• Assay Type:Sandwich
• Sensitivity:5.9 pg/mL
• Standard:1000 pg/mL
• Range:15.63-1000 pg/mL
• Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Length:3.5h
• Research Area:Cytokine;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Family, Member 9(IL1F9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Family, Member 9(IL1F9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Family, Member 9(IL1F9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Interleukin 1 Family, Member 9(IL1F9) in the samples is then determined by comparing the OD of the samples to the standard curve.