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ELK6148-Human GM2A(GM2 Ganglioside Activator) ELISA Kit

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Atleast 7 days from order confirmation date
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  • Detectionrange:0.16-10 ng/mL
  • Alternative Names:SAP-3; Cerebroside Sulfate Activator Protein; Sphingolipid Activator Protein 3
  • Assay Type:Sandwich
  • Sensitivity:0.064 ng/mL
  • Standard:10 ng/mL
  • Sample Type:Serum, plasma, tissue homogenates and other biological fluids
  • Assay Length:3.5h
  • Research Area:Immune molecule;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM2 Ganglioside Activator(GM2A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GM2 Ganglioside Activator(GM2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GM2 Ganglioside Activator(GM2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  GM2 Ganglioside Activator(GM2A) in the samples is then determined by comparing the OD of the samples to the standard curve.