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ELK6159-Human RSPO1(R-Spondin 1) ELISA Kit

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Atleast 7 days from order confirmation date
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  • Detectionrange:15.63-1000 pg/mL
  • Alternative Names:RSPONDIN; Roof plate-specific spondin-1
  • Assay Type:Sandwich
  • Sensitivity:5.5 pg/mL
  • Standard:1000 pg/mL
  • Sample Type:serum, plasma, tissue homogenates and other biological fluids
  • Assay Length:3.5h
  • Research Area:Signal transduction;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 1(RSPO1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to R-Spondin 1(RSPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 1(RSPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  R-Spondin 1(RSPO1) in the samples is then determined by comparing the OD of the samples to the standard curve.