ELK6217-Human GADD45a(Growth Arrest And DNA Damage Inducible Protein Alpha) ELISA Kit

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• Detectionrange:0.16-10 ng/mL
• Alternative Names:DDIT1; GADD45; DNA damage-inducible transcript 1 protein
• Assay Type:Sandwich
• Sensitivity:0.059 ng/mL
• Standard:10 ng/mL
• Sample Type:tissue homogenates, cell lysates and other biological fluids
• Assay Length:3.5h
• Research Area:Signal transduction;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha(GADD45a). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha(GADD45a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Alpha(GADD45a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Growth Arrest And DNA Damage Inducible Protein Alpha(GADD45a) in the samples is then determined by comparing the OD of the samples to the standard curve.