ELK6636-Human WNT11(Wingless Type MMTV Integration Site Family, Member 11) ELISA Kit

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• Detectionrange:0.16-10 ng/mL
• Alternative Names:wnt-11; wnt11; WNT11; wnt11b
• Assay Type:Sandwich
• Sensitivity:0.055 ng/mL
• Standard:10 ng/mL
• Sample Type:tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Length:3.5h
• Research Area:Signal transduction;Metabolic pathway;Bone metabolism;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 11(WNT11). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 11(WNT11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 11(WNT11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Wingless Type MMTV Integration Site Family, Member 11(WNT11) in the samples is then determined by comparing the OD of the samples to the standard curve.