Search Result For ("0")
Whatsapp
Call Us
Login

ELK6682-Human DEFb126(Defensin Beta 126) ELISA Kit

Delivery Time:

Atleast 7 days from order confirmation date
16060.00
  • Detectionrange:7.82-500 pg/mL
  • Alternative Names:ESP13.2; C20orf8; DEFB-26; Epididymal secretory protein 13.2
  • Assay Type:Sandwich
  • Sensitivity:3 pg/mL
  • Standard:500 pg/mL
  • Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Assay Length:3.5h
  • Research Area:Immune molecule;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 126(DEFb126). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Defensin Beta 126(DEFb126). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 126(DEFb126), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Defensin Beta 126(DEFb126) in the samples is then determined by comparing the OD of the samples to the standard curve.

 

 

 

Enquiry