ELK6763-Human PCMT1(Protein L-Isoaspartate-O-Methyltransferase) ELISA Kit

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• Detectionrange:31.25-2000 pg/mL
• Alternative Names:PIMT; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase
• Assay Type:Sandwich
• Sensitivity:13.5 pg/mL
• Standard:2000 pg/mL
• Sample Type:serum, plasma and other biological fluids
• Assay Length:3.5h
• Research Area:Enzyme & Kinase;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein L-Isoaspartate-O-Methyltransferase(PCMT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein L-Isoaspartate-O-Methyltransferase(PCMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein L-Isoaspartate-O-Methyltransferase(PCMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  Protein L-Isoaspartate-O-Methyltransferase(PCMT1) in the samples is then determined by comparing the OD of the samples to the standard curve.