Search Result For ("0")
Whatsapp
Call Us
Login

ELK6895-Human TBX21(T-Box Protein 21) ELISA Kit

Delivery Time:

Atleast 7 days from order confirmation date
16060.00
  • Detectionrange:0.16-10 ng/mL
  • Alternative Names:TBLYM; TBET; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet
  • Assay Type:Sandwich
  • Sensitivity:0.052 ng/mL
  • Standard:10 ng/mL
  • Sample Type:Cell lysates and other biological fluids.
  • Assay Length:3.5h
  • Research Area:Signal transduction;

Test Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21(TBX21). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T-Box Protein 21(TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21(TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  T-Box Protein 21(TBX21) in the samples is then determined by comparing the OD of the samples to the standard curve.

 

 

 

Enquiry