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ELK7649-Mouse OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit

Delivery Time:

Atleast 7 days from order confirmation date
178.00 180.00
  • Detection range:3.13-200 ng/mL
  • Alternative Names:OL
  • Assay Type:Competitive Inhibition
  • Sensitivity:0.89 ng/mL
  • Standard:200 ng/mL
  • Range:
  • Sample Type:serum, plasma and other biological fluids
  • Assay Length:2h
  • Research Area:Metabolic pathway;Endocrinology;Cardiovascular biology;

Test Principle:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Oxidized Low Density Lipoprotein(OxLDL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oxidized Low Density Lipoprotein(OxLDL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of Oxidized Low Density Lipoprotein(OxLDL) in the samples is then determined by comparing the OD of the samples to the standard curve.