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ELK7869-General HA(Histamine) ELISA Kit

Delivery Time:

Atleast 7 days from order confirmation date

Detection range:1.57-100 ng/mL

Alternative Names:Histamin

Assay Type:2h

Sensitivity:0.51 ng/mL

Standard:100 ng/mL


Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length:2h

Research Area:Signal transduction;Infection immunity;

Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Histamine(HA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Histamine(HA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of Histamine(HA) in the samples is then determined by comparing the OD of the samples to the standard curve.