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ELK8267-General DC(Deoxycholate) ELISA Kit

Delivery Time:

Atleast 7 days from order confirmation date

Detection range :15.63-1000 ng/mL

  • Alternative Names :Deoxycholic Acid; Cholanoic Acid; 3a,12a-dihydroxy-5b-cholanate
  • Assay Type :Competitive Inhibition
  • Sensitivity :4.99 ng/mL
  • Standard :1000 ng/mL
  • Range :
  • Sample Type :serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Assay Length :2h
  • Research Area :Endocrinology;      

Test Principle :

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Deoxycholate(DC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Deoxycholate(DC). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of Deoxycholate(DC) in the samples is then determined by comparing the OD of the samples to the standard curve.