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ELK8351-Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit

Delivery Time:

Atleast 7 days from order confirmation date
  • Detection range :0.13-8 ng/mL
  • Alternative Names :15-HETE; 15-Hydroxyeicosatetraenoic Acid)
  • Assay Type :Competitive Inhibition
  • Sensitivity :0.1 ng/mL
  • Standard :8 ng/mL
  • Range :
  • Sample Type :serum, plasma, cell lysates, cell culture supernates and other biological fluids
  • Assay Length :2h
  • Research Area :Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology; 

Test Principle :

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 15-Hydroxyeicosatetraenoic Acid(15-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 15-Hydroxyeicosatetraenoic Acid(15-HETE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of 15-Hydroxyeicosatetraenoic Acid(15-HETE) in the samples is then determined by comparing the OD of the samples to the standard curve.