ELK9461-Human BNIP3L(BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like) ELISA Kit

Delivery Time:

• Detection range:0.16-10 ng/mL
• Alternative Names: BNIP3a; BNIP3H; BNIP3L; NIP3 like protein X; NIP3L; NIX; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• Assay Type: Sandwich
• Sensitivity: 0.094 ng/mL
• Standard: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Assay Length: 3.5h
• Research Area:Infection immunity;

Test Principle:

 The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L) in the samples is then determined by comparing the OD of the samples to the standard curve.