Human HBsAg(HepatitisB surface antigen) ELISA Kit

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• Detection range: 0.31-20 ng/mL
• Alternative Names: Australia antigen
• Assay Type: Sandwich
• Sensitivity: 0.19 ng/mL
• Standard: 20 ng/mL
• Range: 0.31-20 ng/mL
• Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Length: 3.5h
• Research Area: Microbiology；Cancer

Test Principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HepatitisB surface antigen(HBsAg). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HepatitisB surface antigen(HBsAg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HepatitisB surface antigen(HBsAg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of  450nm  ±  10nm. The concentration of  HepatitisB surface antigen(HBsAg) in the samples is then determined by comparing the OD of the samples to the standard curve.