MTB primer and probe have been designed for the specific and exclusive in vitro quantification of MTB Complex [Mycobacterium tuberculosis, Mycobacterium africanum, Mycobacterium bovis, Mycobacterium canettii, Mycobacterium microti.]. The target sequence [MBP64] is highly conserved and has previously been shown to be a good genetic marker for MTB. The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis.