Plasmodium species primer and probe have been designed for the specific and exclusive in vitro detection and genotyping of Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale Plasmodium knowlesi and Plasmodium malariae]. The target sequence [18SrRNA] is highly conserved and has previously been shown to be a good genetic marker for Plasmodium species. The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis.