INTRODUCTION:
Serum Glutamic Oxaloacetate Transaminase (SGOT) also known as Aspartate Aminotransferase (AST) is a tissue enzy me that catalyzes the exchange of amino and keto groups between alpha-amino acids and alpha-keto acids. SGOT is widely distributed in tissue principally cardiac, hepatic, muscle and kidney. Injury to these tissues results in the release of the SGOT (AST) enzyme to general circulation. Following a myocardial infarction, serum levels of SGOT (AST) are elevated and reach a peak in 48 to 60 hours after onset. Hepatobiliary diseases, such as cirrhosis, metastatic carcinoma, and viral hepatitis also will increase serum SGOT levels. The first kinetic assay of SGOT for diagnostic purposes was described by Karmen et al. in 1 955, using a coupled reaction of malate dehydrogenase (MDH) and NADH. This assay system was critically evaluated and optimized in 1960 by Henry et al. In 197 7the International Federation of Clinical Chemistry (IFCC) recommended a reference procedure for the measurement of SGOT activity based upon Karmen's procedures. The SGOT reagent applies the formulation recommended by the IFCC.
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