Product Description:
T4 DNA ligase is a single polypeptide Molecular Weight: 77 kDa (Sedimentation, Lehman 1974) 74 ± 3 kDa (SDS Page, Lehman 1974) 55.3 kDa (Theoretical) Optimal pH: 7.5-8.0 Activators: Requires ATP, Mg2+, and sulfhydryl reagents (DTT, 2-mercaptoethanol) Inhibitors: NaCl (concentrations exceeding 200 mM) Applications: Joining DNA molecules with compatible cohesive termini, or blunt ended double stranded DNA to one another or to synthetic linkers Only T4 DNA ligase joins fragments with overlapping complementary single-stranded protrusions as well as blunt ends Unlike E. coli DNA ligase, T4 enzyme joins duplex DNA molecules at blunt ends Repairs single-stranded nicks in duplex DNA Repairs nicks in duplex DNA, RNA, or DNA/RNA hybrids Ligase chain reaction (LCR)
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