Test principle :
Sensit Leptospira IgM antibody Rapid Test is a lateral flow chromatographic immunoassay. The test strip consists of 1) a pink colored conjugate pad containing Anti-Human IgM conjugated with colloid gold, 2) a nitrocellulose membrane strip pre-coated with recombinant Leptospira Antigen on test line and Goat Anti-Mouse on Control line. Test specimen, with adequate amount of buffer, migrates along the conjugate pad and further across the coated membrane by capillary action. Leptospira IgM antibodies present in the sample, complex with the Anti-Human IgM present in the conjugate pad and gets captured onto the coated Leptospira antigen. Thus giving a colored test band, indicating a positive test result. If the respective antibody is not present in the sample, no reaction occurs and no test line is formed. The sample then migrates further along the strip until it reaches the control band, where excess Anti-Human IgM-CGC gets bound and produces a second visible line on the membrane. This control line indicates that the sample has migrated across the membrane as intended
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